THE COMBINED EFFECT OF NONSTEROIDAL ANTI‐OESTROGENS AND SEX STEROIDS ON THE GROWTH OF RAT UTERINE ADENOCARCINOMA CELLS IN TISSUE CULTURE

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Abstract
Nonsteroidal anti‐oestrogenic drugs, tamoxifen and clomiphene citrate, at concentrations higher than 0.001 μg/ml reduced the colony forming ability of cells derived from a rat uterine adenocarcinoma in vitro. The 50 per cent inhibitory dose of these drugs was about one‐hundredth of that of sex steroids. When the cells were treated with combinations of these nonsteroidal anti‐oestrogenic drugs and the 50 per cent inhibitory dose (8 μg/ml) of progesterone, a synergistic effect on the inhibition of colony formation was observed. In contrast to progesterone, oestradiol‐17β (the 50 per cent inhibitory dose of which was about 16 μg/ml) suppressed additively the colony formation only in combination with low doses of anti‐oestrogenic drugs.