Electron Microscopic Observation of the Sporangial Structure of an Actinomycete, Microellobosporia flavea

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Abstract
Ultrathin sections of sporangia of the actinomycete Microellobosporia flavea were examined with an electron microscope. The sporangial wall is a thin wrinkled membrane which seems to be an extension of the outer layer of the cell wall of the sporangiosphore. A substance (probably a liquid) is located between the sporangial wall and the sporangiospores. The spores are ovoid and consist of a laminated wall in which two layers can be differentiated. The wall is 30-40 m[mu] thick but thickens to 90-120 m[mu] at the point of contact between two spores. Inside the spores one can differentiate a finely granular nucleus, large vacuoles which are probably filled with a fatty substance and a coarsely granular cytoplasm. The cytology of the sporangiospores of M. flavea is similar to that of the conidia of Waksmania rosea.

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