Isoproterenol-induced cardiac lesions in frog observed in vivo

Publisher Website
Google Scholar
Abstract
In some winter (October–February) frogs (R. pipiens) that died at a high environmental temperature (+29 °C) the cardiac ventricle showed aneurysm-like formations. An attempt was made to produce a similar type of cardiac lesion in living animals. Frogs kept at +25 °C for 3 days and injected simultaneously with isoproterenol (150–200 mg/kg body weight twice in 48 h) showed various types of cardiac lesions in vivo: (i) initial stage, (ii) blisters, (iii) aneurysm-like formations. Preliminary microscopic findings showed large intramural hematomas in the cardiac wall where in vivo aneurysm-like formations were observed. No stromatic reaction occurred in freshly killed specimens. These lesions observed in the living beating heart in situ showed the following: (a) paradoxical movement of aneurysms, (b) size and shape dependent on intraventricular pressure, (c) shifts from one to another type of lesion, (d) a certain degree of reversibility, (e) simultaneous occurrence in the same heart of many types of injury. Typical localization of the aneurysm-like formations was observed: most were situated in the left and right upper corner and at the apex of the ventricle. This type of experimentally produced cardiac lesion can be studied in detail, the general circulation being intact for hours in the beating heart, provided suitable conditions (temperature and humidity) are preserved. This living and dynamic model of cardiac lesions is open to further experimental research: hemodynamic, pharmacodynamic, and ultrastructural.

This publication has 0 references indexed in Scilit: