Prostaglandin E2 excretion, urine flow and papillary osmolality during saline or dextrose infusion in the conscious rat.

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Abstract
Conscious rats received infusions at 5.8 ml/h of either 0.9% NaCl or 5% dextrose, via a tail vein, for 6 h. During this infusion period, urine was collected from the animals, and the urine volume, Na concentration and immunoreactive PGE2 [prostaglandin E2] were determined. Urine flow in both groups was stable during the 2-6 h period of the infusion and was not significantly different between the 2 groups. Na output was also stable over the 2-6 h infusion period, but obviously the output of the saline-infused group was higher than that of the dextrose-infused group. Urianry PGE2 output was not significantly different between the groups in the 2-4 h period (79.4 .+-. 8.6 pmol/2 h in the saline-infused group, 82.1 .+-. 5.7 pmol/2 h in the dextrose-infused group). In the 4-6 h period, PGE2 output remained at this level (82.0 .+-. 7.8 pmol/2 h) in the dextrose-infused group, but fell significantly (to 53.7 .+-. 5.0 pmol/2 h) in the saline-infused group. In separate groups of animals which received saline or dextrose infusions as above, renal papillary osmolality was determined. The osmolality was significantly (P < 0.001) higher in the saline-infused group. Renal PGE2 synthesis is unlikely to be directly involved in Na homeostasis and that PGE2 synthesis as measured by urinary PGE2 excretion is not controlled by the papillary osmolality.