Coordinated mRNA and Protein Expression of Human LAMP‐1 in Induction of Melanogenesis After UV‐B Exposure and Co‐Transfection of Human Tyrosinase and TRP‐1 cDNAs
- 1 October 1994
- journal article
- Published by Wiley in Pigment Cell Research
- Vol. 7 (5) , 311-319
- https://doi.org/10.1111/j.1600-0749.1994.tb00633.x
Abstract
In order to better understand the cascade of melanogenic events in melanocytes, this report has introduced our two recent approaches for the expression of melanogenesis/or melanosome-associated genes and encoded proteins in melanocytes (melanoma cells) after repeated exposure to UV-B and after cotransfection of two human genes, i.e., tyrosinase and tyrosinase-related protein-1 (TRP-1). Repeated exposure of UV-B (2.5-5.0 mJ/cm2) caused not only upregulation of tyrosinase and TRP-1 genes but also coordinated increase in the gene and protein synthesis expression of Lamp-1 (lysosome-associated membrane protein-1). When COS-7 kidney cells and amelanotic melanoma (C32 and SK-MEL-24) and melanotic melanoma (G361 and SK-MEL-23) cells were exposed to cotransfection of human tyrosinase and TRP-1 cDNAs, there was also an increased expression of Lamp-1 mRNA and protein along with tyrosinase activation and new melanin synthesis. Importantly, single transfectants of human tyrosinase cDNA revealed marked cellular degeneration, whereas this degeneration was not seen in single transfectants of TRP-1 cDNA or cotransfectants of human tyrosinase and TRP-1 cDNAs, indicating that TRP-1 prevented, along with Lamp-1, programmed death of melanocytes after transfection of tyrosinase gene. The coordinated expression of TRP-1 and Lamp-1 was further confirmed by antisense oligodeoxynucleotide hybridization experiment against Lamp-1 gene, showing the decreased expression of TRP-1 as identified by three different types of anti-TRP-1 monoclonal antibodies.(ABSTRACT TRUNCATED AT 250 WORDS)Keywords
This publication has 32 references indexed in Scilit:
- Cotransfection of Genes Encoding Human Tyrosinase and Tyrosinase-Related Protein-1 Prevents Melanocyte Death and Enhances Melanin Pigmentation and Gene Expression of Lamp-1Experimental Cell Research, 1994
- Role of Gene Expression and Protein Synthesis of Tyrosinase, TRP-1, Lamp-1, and CD63 in UVB-Induced Melanogenesis in Human MelanomasJournal of Investigative Dermatology, 1994
- Identification of a cDNA Coding for a Ca2+-Binding Phosphoprotein (p90), Calnexin, on Melanosomes in Normal and Malignant Human MelanocytesExperimental Cell Research, 1993
- Pigmentation Genes: the Tyrosinase Gene Family and the pmel 17 Gene Family.Journal of Investigative Dermatology, 1993
- The murine pallid mutation is a platelet storage pool disease associated with the protein 4.2 (pallidin) geneNature Genetics, 1992
- Light is a dominant mouse mutation resulting in premature cell deathNature Genetics, 1992
- Leukotrienes C4 and D4 As Potent Mitogens for Cultured Human Neonatal MelanocytesJournal of Investigative Dermatology, 1989
- Basic fibroblast growth factor from human keratinocytes is a natural mitogen for melanocytes.The Journal of cell biology, 1988
- Prostaglandin E2 and D2 but Not MSH Stimulate the Proliferation of Pigment Cells in the Pinnal Epidermis of the DBA/2 MouseJournal of Investigative Dermatology, 1986
- ULTRASTRUCTURAL AND CYTOCHEMICAL OBSERVATIONS ON B-16 AND HARDING-PASSEY MOUSE MELANOMAS THE ORIGIN OF PREMELANOSOMES AND COMPOUND MELANOSOMESJournal of Histochemistry & Cytochemistry, 1968