The refractometric determination of the total protein concentration in some animal plasmas

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Abstract
Extract Refractometry has been used for serum or plasma total, protein determination since the beginning of this century and while at times the value and accuracy of the technique has been open to question (see Lines and Raine, 1970a Babul, J. and Stellwagen, E. 1969. Measurement of protein concentration with interference optics. Analyt. Biochem., 28: 216–221. [Crossref], [PubMed], [Web of Science ®] [Google Scholar] , b Barry, K. G. , McLaurin, A. W. and Parnell, B. L. 1960. A practical temperature-compensated hand refractometer (the TS-meter). Its clinical use and application in estimation of total serum proteins. J. lab. din. Med., 55: 803–808. [Google Scholar] ), many studies have shown close agreement between refractometric determination of serum total protein and estimation by such techniques as the Biuret method and the Kjeldahl nitrogen determination (Lines and Raine, 1970b Drickman, A. and McKeon, F. A. 1962. Determination of total serum protein by means of the refractive index of serum. Am. f. clin. Path., 38: 392–396. [Google Scholar] ; Barry et al., 1960 Harboe, M. 1959. A method for determination of haemoglobin in plasma by near-ultraviolet spec-trophotometry. Scand, J. clin. lab. Invest., 11: 66–70. [Taylor & Francis Online] [Google Scholar] ; Drickman and McKeon, 1962 Henry, R. J. 1974. Clinical Chemistry, 177–177. New York, Evanston and London: Harper & Row. John Weatherhill, Tokyo [Google Scholar] ).

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