Evaluation of a Dye-binding Method for Determination of Serum Albumin
- 1 March 1965
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 11 (3) , 441-447
- https://doi.org/10.1093/clinchem/11.3.441
Abstract
A simple dye-binding method for determining serum albumin, which employs the dye, 2-(4'-hydroxybenzeneazo) benzoic acid (HBABA), is described. Albumin in 0.1 ml. of serum is determined colorimetrically at 510 mµ. Reliability of the method has been established by comparison with another method using HBABA and with a salt fractionation-biuret method, by recovery experiments, variation in ambient temperature, demonstration of optimal dye molarity and pH, and replicate analyses.Keywords
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