Steady-state parameters of an enzyme from n.m.r. spin transfer with thermal variation
- 15 May 1987
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 244 (1) , 247-248
- https://doi.org/10.1042/bj2440247
Abstract
N.m.r. spin-exchange analysis of enzymic reactions at chemical equilibrium is akin to radioactive-tracer-exchange analysis; unidirectional flux rates are obtained for the overall reaction. These data, by themselves, are not sufficient to define the values of all the individual rate constants or steady-state parameters. However, it is shown that, by measuring the dependence of the exchange rate constants on solute concentration and temperature, the individual rate constants, and hence the steady-state parameters, can be obtained for a simple enzyme system.This publication has 7 references indexed in Scilit:
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