Studies on the Regeneration of Protochlorophyllide after Brief Illumination of Etiolated Bean Leaves

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Abstract
The effects of various inhibitors of nucleic acid and protein synthesis on protochlorophyllide synthesis in darkgrown Phaseolus vulgaris var. Red Kidney have been studied. Actinomycin D, chloramphenicol, and puromycin inhibit the regeneration of protochlorophyllide holochrome (detected as a 650 m [mu] absorption peak) in vivo in darkness after photoconversion of endogenous protochlorophyllide a to chlorophyllide a; this inhibition does not occur in similarly treated leaves supplied with [delta]-aminolevulinic acid. These data suggest that the regeneration of protochlorophyllide results from the synthesis of RNA and enzymes required for the production of [delta] -aminolevulinate.