The Effect of a Shift on the Frequency of Transduced Salmonella typhimurium Cells in the Early Period of Transduction

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Abstract
The frequency of transduced cells (gal+) was reduced by the transfer of the Salmonella typhimurium recipient cells with adsorbed P22 transducing phage from a complete into a minimal medium. This reduction was most conspicuous immediately after the adsorption of the phage (3 min.) and then gradually decreased as transfer was delayed. After cultivation for 90 min. under conditions which permitted growth and multiplication of the cells the frequency of the transduced cells was practically no more influenced. The sensitivity of the potentially transduced cells to acriflavine was assayed soon after the adsorption of the phage. An early acriflavine-reversible phase was demonstrated in more than 50% of transductions. A close time relationship was found between shift-reversible and acriflavine-reversible phases.

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