Evidence for a mediator cycle at the initiation of transcription
Open Access
- 10 June 1997
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 94 (12) , 6075-6078
- https://doi.org/10.1073/pnas.94.12.6075
Abstract
Free and elongating (DNA-bound) forms of RNA polymerase II were separated from yeast. Most cellular polymerase II was found in the elongating fraction, which contained all enzyme phosphorylated on the C-terminal domain and none of the 15-subunit mediator of transcriptional regulation. These and other findings suggest that mediator enters and leaves initiation complexes during every round of transcription, in a process that may be coupled to C-terminal domain phosphorylation.Keywords
This publication has 21 references indexed in Scilit:
- Identification of Rox3 as a Component of Mediator and RNA Polymerase II HoloenzymePublished by Elsevier ,1997
- The RNA polymerase II general elongation factorsTrends in Biochemical Sciences, 1996
- A Minimal Set of RNA Polymerase II Transcription Protein InteractionsPublished by Elsevier ,1996
- TheKIN28Gene is Required both for RNA Polymerase II Mediated Transcription and Phosphorylation of the Rpb1p CTDJournal of Molecular Biology, 1995
- A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase IICell, 1994
- An RNA polymerase II holoenzyme responsive to activatorsNature, 1994
- A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeastCell, 1993
- CTD kinase associated with yeast RNA polymerase II initiation factor bCell, 1991
- A mediator required for activation of RNA polymerase II transcription in vitroNature, 1991
- A novel mediator between activator proteins and the RNA polymerase II transcription apparatusCell, 1990