(N-Succinimidyl 4-pentynoate)(hexacarbonyldicobalt): a transition-metal carbonyl complex having similar uses to the Bolton-Hunter reagent

1 January 1991

journal article
Published by American Chemical Society (ACS) in Bioconjugate Chemistry

Vol. 2 (1) , 13-15
https://doi.org/10.1021/bc00007a002

Abstract

The synthesis of the transition-metal carbonyl complex (N-succinimidyl 4-pentynoate)hexacarbonyldicobalt [[(C4H4O2N)O(CO)CH2CH2C identical to CH]Co2(CO)6] is described. This cobalt carbonyl complex is structurally similar to the Bolton-Hunter conjugation reagent and has been successfully employed as a nonradioactive tracer for labeling the drug carbamazepine. The metal carbonyl tracer can be detected at extremely low concentrations (ca. 1 pmol) by FT-IR spectroscopy in the v(CO) region (2150-1800 cm-1). The cobalt carbonyl labeled carbamazepine retains good recognition for anti-carbamazepine antibodies. This novel labeling procedure, which can be broadly termed carbonylmetalloimmunoassay (CMIA), has considerable potential for assaying a wide range of biological materials.

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