Statistical analysis of NADH oscillations in the yeastsaccharomyces carlsbergensisfermenting on different sugars

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Abstract
In order to detect hidden periodicities in glycolytic oscillations and to estimate period lengths the interactive program system TIMESDIA was applied. This program system contains a collection of mathematical procedures apt to the above purpose. The period length of glycolytic oscillations in a suspension of yeast cells depends on the kind of sugar which is fermented by the cells: with each of the four sugars D‐gluctose, D‐fructose, D‐mannose and sucrose different period lengths are obtained. These oscillations are nearly sinusoidal, since in a fourier expansion of the signals the first term exceeds the following ones by at least one order of magnitude. If mixtures of D‐glucose and D‐fructose are added to a cell suspension, the resulting period lengths are between the values which are characteristic for both sugars alone. These oscillations are monotonic too, they are not a superposition of two oscillations different in period length and amplitude, as was shown by the application of our program system. Thus, if there exist two different oscillators at all (one for each sugar), there must be a strong coupling between them. The influence of D‐fructose on the kinetics of phosphofructokinase as it has been published recently, supports the assumption that the glycolytic oscillator itself (i.e. phosphofructokinase and the adenylates) is influenced differently by several sugars and sugar phosphates depending on their concentrations within the cell.

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