Poly 2'-O-ethylcytidylate, an inhibitor and poor template for AMV reverse transcriptase
Open Access
- 1 June 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (6) , 1603-1612
- https://doi.org/10.1093/nar/3.6.1603
Abstract
Poly(2′-O-ethylcytidylate) is a poor template-primer for purified avian myeloblastosis virus reverse transcriptase; the relative activities of the template-primers poly(C)·oligo(dG), poly(Cm)·oligo(dG) are poly(Ce)·oligo(dG) are 23:16:1. A mixture of poly(Ce) and poly(dI) is inactive as template-primer, in agreement with the observed inability of these to form a helical complex. By contrast the inactivity of poly(Ce)·poly(I) is shown to be due to the influence of the 2′-O-ethyl residue. Poly(Ce) inhibits poly(A)·oligo(dT)-directed polymerase activity, with Ki = 3 μM, but marked inhibition with poly(A)·poly(dT) occurs only at low concentrations of the latter. Poly(Ce) did not inhibit template-primer activity of poly(c)·poly(dI) and poly(dC)·poly(dI). Qualitative physico-chemical studies show only partial complex formation between oligo(dG) and poly(c) and its 2′-O-alkyl analogues. This is discussed in relation to the widespread use of poly(c)·oligo(dG) as a template-primer for reverse transcriptase.Keywords
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