Frozen Vegetables

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Abstract
72 glass containers of frozen peas, 24 uninoculated, 24 lightly inoculated, and 24 heavily inoculated, were examined. No toxin developed in peas examined immediately after defrosting, and none developed in those defrosted and held for 3 days in the ice-box. Toxin was obtained from the spoiled peas in 1 of the 24 uninoculated containers, and Clostridium botulinus cultures were recovered from 8. The spoiled contents of 3 of the 24 lightly inoculated containers were toxic, and cultures were obtained (presumptive identification) from 11. The 24 heavily inoculated containers showed 5 to be toxic after spoilage, and the organism was recovered (presumptive identification) from every container. All the toxic containers showed type B toxin. Of the organisms recovered from uninoculated containers, 7 were type A and 1 was type B.

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