Improved Method for Measurement of delta-Aminolevulinic Acid Dehydratase Activity of Human Erythrocytes
Open Access
- 1 October 1971
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 17 (10) , 1038-1041
- https://doi.org/10.1093/clinchem/17.10.1038
Abstract
We have evaluated and modified a current method for estimating δ-aminolevulinic acid dehydratase (5-aminolaevulinate hydro-lyase, EC 4.2.1.24) activity in blood, so as to adequately control the pH of the enzyme-substrate solution at the optimum throughout the incubation period. This has improved the sensitivity and reliability of the assay. Also, N-ethylmaleimide has been substituted for a potentially hazardous mercury salt, used to remove sulfhydryl groups before color development with a modified Ehrlich’s reagent.Keywords
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