The relationship between purines, pyrimidines, nucleosides, and glutamine for fibroblast cell proliferation

Publisher Website
Google Scholar
Abstract
Gln is a critical requirement for cell proliferation in vitro. Depletion of Gln from the culture medium supporting growing [3T3] cells significantly reduced the proportion of cells undergoing DNA synthesis. Similarly Gln depletion significantly reduced the stimulatory response of quiescent cells to 10% serum. The inhibitory effects of depletion of Gln.sbd.in either of these 2 situations.sbd.can be overcome by the addition of adenine or adenosine. Adenine was the only nitrogen base and adenosine was the only nucleoside for which this effect was observed. Such effects could also be achieved by addition of the purine metabolites hypoxanthine and inosine. Gln (or adenine/adenosine) is only required during a limited interval coinciding with the late part of the G1-phase and the beginning of S-phase. Gln may exert its main regulatory effects on cell prolifertion by acting as a pecursor for adenine and adenosine.