Transcription inLactobacillaceae.DNA-Dependent RNA Polymerase fromLactobacillus caseiIsolation of Transcription Factor y
- 1 January 1977
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 358 (2) , 1093-1104
- https://doi.org/10.1515/bchm2.1977.358.2.1093
Abstract
Purified DNA-dependent RNA polymerase [EC 2.7.7.6] from L. casei shows a subunit pattern similar to that of other prokaryotic RNA polymerases. A polypeptide .gamma. (MW = 28,000) with unknown function is tightly bound to about half of the polymerase molecules. A 2nd additional polypeptide (MW = 80,000), already known from L. curvatus, is only present in a fraction of the polymerase molecules. It stimulates transcription of holoenzyme on phage DNA about twice. An isolation procedure for native y is described.This publication has 7 references indexed in Scilit:
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