Solvent System Design for Silica Gel Liquid Chromatography of Digitalis Glycosides. Determination of Digoxin in Pharmaceutical Preparation
- 1 January 1984
- journal article
- research article
- Published by Pharmaceutical Society of Japan in YAKUGAKU ZASSHI
- Vol. 104 (5) , 454-457
- https://doi.org/10.1248/yakushi1947.104.5_454
Abstract
Solvent system optimization for digitalis glycosides was examined by silica gel normal-phase liquid chromatography. 2-Propanol was added to a diluent, n-hexane, for increasing the solubility of water which was a fine modifier for reducing peak tailing. Ethanol was incorporated into the carrier solvent as a strong component to control the retentivity. As a systematically designed 4-component phase system, n-hexane-2-propanol-ethanol-water (52:20:25:3 v/v [vol/vol]) was applied to the high performance liquid chromatographic analysis of digoxin in commercially available tablet preparations, using the clean-up procedure. The phase system showed preferable selectivity for the simultaneous resolution of 3 digitalis glycosides.Keywords
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