Crystal structure of human angiogenin reveals the structural basis for its functional divergence from ribonuclease.
Open Access
- 12 April 1994
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (8) , 2915-2919
- https://doi.org/10.1073/pnas.91.8.2915
Abstract
Angiogenin, a potent inducer of neovascularization, is the only angiogenic molecule known to exhibit ribonucleolytic activity. Its overall structure, as determined at 2.4 A, is similar to that of pancreatic ribonuclease A, but it differs markedly in several distinct areas, particularly the ribonucleolytic active center and the putative receptor binding site, both of which are critically involved in biological function. Most strikingly, the site that is spatially analogous to that for pyrimidine binding in ribonuclease A differs significantly in conformation and is "obstructed" by glutamine-117. Movement of this and adjacent residues may be required for substrate binding to angiogenin and, hence, constitute a key part of its mechanism of action.Keywords
This publication has 32 references indexed in Scilit:
- Crystal structure of cyclin-dependent kinase 2Nature, 1993
- Crystallization and preliminary X-ray analysis of human angiogeninJournal of Molecular Biology, 1992
- A covalent angiogenin/ribonuclease hybrid with a fourth disulfide bond generated by regional mutagenesisBiochemistry, 1989
- Isolation of angiogenin from normal human plasmaBiochemistry, 1987
- Characteristic ribonucleolytic activity of human angiogeninBiochemistry, 1986
- Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cellsBiochemistry, 1985
- Nuclear magnetic resonance and neutron diffraction studies of the complex of ribonuclease A with uridine vanadate, a transition-state analogBiochemistry, 1985
- Dictionary of protein secondary structure: Pattern recognition of hydrogen‐bonded and geometrical featuresBiopolymers, 1983
- Crystal structure of cat muscle pyruvate kinase at a resolution of 2.6 ÅJournal of Molecular Biology, 1979
- The structure of cytidilyl(2′,5′)adenosine when bound to pancreatic ribonuclease SJournal of Molecular Biology, 1977