A Simple High Performance Liquid Chromatography Procedure for the Determination of N1-Methylnicotinamide in Urine
- 1 May 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 7 (5) , 969-977
- https://doi.org/10.1080/01483918408074019
Abstract
A simple high performance liquid chromatography procedure for the determination of N1-methylnicotinamide in urine samples is described. The procedure eliminates the need for extraction or ion exchange clean-up of urine samples prior to their analysis. Human and rat urine samples can be analyzed for N1-methylnicotinamide directly following a simple pH adjustment. The metabolite was separated and quantitated on a 5.mu. Ultrasphere ODS (C18) reverse-phase column. The mobile phase contained 10 mM K2HPO4 and 10 mM sodium 1-octanesulfonate in 8% acetonitrile at pH 7.0. The system was used to conduct over 1000 determinations during a period of 3 mo. without reduction in performance or efficiency.This publication has 3 references indexed in Scilit:
- Quantitation of urinary niacin metabolites by reversed-phase liquid chromatographyThe American Journal of Clinical Nutrition, 1982
- Determination of nicotinamide in human plasma and urine by ion-pair reversed-phase high-performance liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1980