Prenatal fetal sex diagnosis by detecting amelogenin gene in maternal plasma
- 20 July 2005
- journal article
- research article
- Published by Wiley in Prenatal Diagnosis
- Vol. 25 (7) , 577-581
- https://doi.org/10.1002/pd.1192
Abstract
Objectives To provide a new, reliable noninvasive method for fetal sex determination. Methods Fetal sex was detected in 32 early pregnant women by identifying the amelogenin gene in maternal plasma using nested PCR analysis. First, the 122/128 bp of X‐Y homologous region containing 6 bp deletions in the intron 3 of amelogenin gene in X chromosome was amplified, and then the nested PCR was carried out, whose 3′ end of the upstream primer is just located in the deletion region. The fetus was male or female, depending on whether it had the 89‐bp nested PCR product or not. Results The 89 bp of nested PCR product was detected in 19 plasma samples obtained from pregnant women, deducing they bear the male fetus and the remaining pregnant women bear female. When compared with the birth outcome, two samples were pseudo‐positive. The coincidence was 93.8%. This method had high sensitivity that even trace amount of target fetal DNA (10 pg) could be detected. Conclusions This conventional nested PCR analysis of amelogenin gene promises to be a reliable method for noninvasive fetal sex determination at early pregnancy using maternal plasma DNA. Copyright © 2005 John Wiley & Sons, Ltd.Keywords
This publication has 23 references indexed in Scilit:
- Size Distributions of Maternal and Fetal DNA in Maternal PlasmaClinical Chemistry, 2004
- Exo-Proofreading, A Versatile SNP Scoring TechnologyGenome Research, 2003
- Huntington disease–unaffected fetus diagnosed from maternal plasma using QF‐PCRPrenatal Diagnosis, 2003
- Replicate real‐time PCR testing of DNA in maternal plasma increases the sensitivity of non‐invasive fetal sex determinationPrenatal Diagnosis, 2003
- Prenatal detection of a cystic fibrosis mutation in fetal DNA from maternal plasmaPrenatal Diagnosis, 2002
- Fetal gender determination in early pregnancy through qualitative and quantitative analysis of fetal DNA in maternal serumHuman Genetics, 2001
- Detection of Y chromosome‐specific DNA in the plasma and urine of pregnant women using nested polymerase chain reactionPrenatal Diagnosis, 2001
- Presence of fetal DNA in maternal plasma and serumThe Lancet, 1997
- Sex identification of forensic specimens by polymerase chain reaction (PCR): Two alternative methodsForensic Science International, 1991
- PRENATAL SEX DETERMINATION BY DNA AMPLIFICATION FROM MATERNAL PERIPHERAL BLOODThe Lancet, 1989