High-Level Expression and Secretion of Methyl Parathion Hydrolase in Bacillus subtilis WB800
Open Access
- 1 July 2005
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 71 (7) , 4101-4103
- https://doi.org/10.1128/aem.71.7.4101-4103.2005
Abstract
The methyl parathion hydrolase (MPH)-encoding gene mpd was placed under the control of the P43 promoter and Bacillus subtilis nprB signal peptide-encoding sequence. High-level expression and secretion of mature, authentic, and stable MPH were achieved using the protease-deficient strain B. subtilis WB800 as the host.Keywords
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