Design of camp–CRP‐activated promoters in Escherichia coli
- 1 February 1991
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 5 (2) , 433-437
- https://doi.org/10.1111/j.1365-2958.1991.tb02126.x
Abstract
We have studied the doeP2 promoter of Escherichia coli to define features that are required for optimal activation by the complex of adenosine 3′, 5′ monophosphate (cAMP) and the cAMP receptor protein (CRP). Systematic mutagenesis of deoP2 shows that the distance between the CRP site and the ‐10 hexamer is the crucial factor in determining whether the promoter is activated by camp–CRP. Based on these observations, we propose that camp–CRP‐activated promoters can be created by correctly aligning a CRP target and a ‐ 10 hexamer. This idea has been successfully tested by converting both a CRP‐in‐dependent promoter and a sequence resembling the consensus ‐10 hexamer to strongly camp–CRP‐activated promoters.Keywords
This publication has 27 references indexed in Scilit:
- Semisynthetic promoters activated by cyclic AMP receptor protein of Escherichia coliGene, 1989
- CRP/cAMP‐ and CytR‐regulated promoters in Escherichia coli K12: the cdd promoterMolecular Microbiology, 1989
- Transcription from the Escherichia coli melR promoter is dependent on the cyclic AMP receptor proteinGene, 1988
- Rapid chemical probing of conformation in 16 S ribosomal RNA and 30 S ribosomal subunits using primer extensionJournal of Molecular Biology, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- DNA Sequences Involved in Expression and Regulation of deoR-, cytR- and cAMP/CRP-Controlled Genes in Escherichia ColiPublished by Springer Nature ,1985
- Cyclic AMP Receptor Protein: Role in Transcription ActivationScience, 1984
- The locus of sequence-directed and protein-induced DNA bendingNature, 1984
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Binding of RNA polymerase and the catabolite gene activator protein within the cat promoter in Escherichia coliJournal of Molecular Biology, 1981