Identification and characterisation of constitutional chromosome abnormalities using arrays of bacterial artificial chromosomes
Open Access
- 17 February 2004
- journal article
- Published by Springer Nature in British Journal of Cancer
- Vol. 90 (4) , 860-865
- https://doi.org/10.1038/sj.bjc.6601588
Abstract
Constitutional chromosome deletions and duplications frequently predispose to the development of a wide variety of cancers. We have developed a microarray of 6000 bacterial artificial chromosomes for array-based comparative genomic hybridisation, which provides an average resolution of 750 kb across the human genome. Using these arrays, subtle gains and losses of chromosome regions can be detected in constitutional cells, following a single overnight hybridisation. In this report, we demonstrate the efficiency of this procedure in identifying constitutional deletions and duplications associated with predisposition to retinoblastoma, Wilms tumour and Beckwith–Wiedemann syndrome.Keywords
This publication has 33 references indexed in Scilit:
- Characterization of the 1p/19q Chromosomal Loss in Oligodendrogliomas Using Comparative Genomic Hybridization Arrays (CGHa)Journal of Neuropathology and Experimental Neurology, 2004
- Definition of a Critical Region on Chromosome 18 for Congenital Aural Atresia by ArrayCGHAmerican Journal of Human Genetics, 2003
- Hidden gene amplifications in aggressive B-cell non-Hodgkin lymphomas detected by microarray-based comparative genomic hybridizationOncogene, 2003
- Severe congenital Factor VII deficiency associated with the 13q deletion syndromeAmerican Journal of Hematology, 2002
- High-Throughput Analysis of Subtelomeric Chromosome Rearrangements by Use of Array-Based Comparative Genomic HybridizationAmerican Journal of Human Genetics, 2002
- Integration of cytogenetic landmarks into the draft sequence of the human genomeNature, 2001
- Neural tube defects and the 13q deletion syndrome: Evidence for a critical region in 13q33-34American Journal of Medical Genetics, 2000
- The aniridia-Wilms' tumour association: molecular and genetic analysis of chromosome deletions on the short arm of chromosome 11Human Genetics, 1989
- Effect of the esterase-D phenotype on its in vitro enzyme activityHuman Genetics, 1986
- Assignment of the genes for human mitochondrial malate dehydiogenase to chromosome 7, for mannose phosphate isomerase and pyruvate kinase to chromosome 15, and, probably, for human esterase-D to chromosome 13 using man-mouse hybridsCytogenetic and Genome Research, 1975