Identification of the mcrC gene product in Methanococcus vannielii

15 July 1993

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 111 (1) , 129-134
https://doi.org/10.1016/0378-1097(93)90193-6

Abstract

The polypeptide encoded by the mcrC gene has been identified in Methanococcus vannielii by immunoblotting using rabbit antibodies raised against the product of a lacZ-mcrC gene fusion synthesized and purified from Escherichia coli. The mcrC gene product (gpmcrC) was located in both supernatant and pellet fractions after centrifugation of Mc. vannielii cell extracts for 2 h at 100 000 ×g. When anaerobic reducing conditions were maintained during purification, gpmcrC co-sedimented through sucrose gradients to the same position as molecules of the methyl coenzyme M reductase holoenzyme (approx. 300 kDa). This co-sedimentation was lost under aerobic, nonreducing conditions.

Keywords

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