Phytochelatin Synthesis and Glutathione Levels in Response to Heavy Metals in Tomato Cells
Open Access
- 1 December 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 85 (4) , 1031-1035
- https://doi.org/10.1104/pp.85.4.1031
Abstract
Cell suspension cultures of tomato, Lycopersicon esculentum Mill. cv VFNT-Cherry, produce phytochelatins (poly[γ-glutamylcysteinyl]glycines) when exposed to cadmium. The synthesis of these peptides is accompanied by a decline in cellular levels of glutathione. Buthionine sulfoximine, an inhibitor of glutathione synthesis, inhibits the sustained production of phytochelatins. However, phytochelatin synthesis can occur in the presence of buthionine sulfoximine provided that sufficient glutathione is available. These results indicate that glutathione is a substrate for phytochelatin synthesis. The protein synthesis inhibitor cycloheximide does not affect the initial production of phytochelatin.Keywords
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