Regulation of ribonucleic acid synthesis in spheroplasts, cold-shocked cells, and toluene-treated cells of Escherichia coli

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Abstract
The stringent control of rRNA synthesis by removal of cell wall, cold-shock treatment of cells, LiCl treatment of toluene-treated cells and hypotonic treatment of spheroplasts was examined using E. coli rel+. The removal of cell wall by penicillin, lysozyme or cold-shock treatment affected stringent control. The stringent control disappeared after LiCl treatment of the toluenized cells with the release of some protein component(s) possibly from the cytoplasmic membrane. Hypotonic and other treatments of spheroplasts disrupting the cytoplasmic membrane also led to the abolishment of stringent control. The operation of the stringent control of rRNA synthesis probably requires cytoplasmic membrane, where some proteins sensitive to LiCl treatment are embedded.