Frequency of Mutation to Rifampin Resistance in Streptococcus pneumoniae Clinical Strains: hexA and hexB Polymorphisms Do Not Account for Hypermutation
Open Access
- 1 April 2003
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 47 (4) , 1464-1467
- https://doi.org/10.1128/aac.47.4.1464-1467.2003
Abstract
The frequency of mutation to rifampin resistance of 200 clinical Streptococcus pneumoniae isolates was examined. Two peaks were observed in the distribution, with mode frequencies of 2.5 × 10−7 (20% of isolates) and 2.5 × 10−8. The hexA and hexB gene entire sequences were analyzed in 13 isolates. Sequences from both hypermutable and “normomutable” strains were conserved relative to that of the R6 S. pneumoniae control strain. The phenotypic Hex system proficiency, in terms of transforming efficiency, was also maintained irrespective of the variations in mutation frequency values.Keywords
This publication has 18 references indexed in Scilit:
- Response of Escherichia coli hypermutators to selection pressure with antimicrobial agents from different classesJournal of Antimicrobial Chemotherapy, 2002
- Very Low Cefotaxime Concentrations Select for Hypermutable Streptococcus pneumoniae PopulationsAntimicrobial Agents and Chemotherapy, 2002
- Hyperrecombination in Streptococcus pneumoniae Depends on an Atypical mutY HomologueJournal of Bacteriology, 2000
- High Frequency of Hypermutable Pseudomonas aeruginosa in Cystic Fibrosis Lung InfectionScience, 2000
- Population biology of Streptococcus pneumoniae isolated from oropharyngeal carriage and invasive disease The GenBank accession numbers for the sequences of the trpA/B alleles determined in this study are AF157817 to AF157826.Microbiology, 1999
- Development of competence in Streptococcus pneumoniae: pheromone autoinduction and control of quorum sensing by the oligopeptide permeaseMolecular Microbiology, 1998
- Control of Recombination Rate During Transformation ofStreptococcus pneumoniae: An OverviewMicrobial Drug Resistance, 1997
- Homeologous recombination and mismatch repair during transformation in Streptococcus pneumoniae: saturation of the Hex mismatch repair system.Proceedings of the National Academy of Sciences, 1995
- Characterization of the mutX gene of Streptococcus pneumoniae as a homologue of Escherichia coli mutT, and tentative definition of a catalytic domain of the dGTP pyrophosphohydroiasesMolecular Microbiology, 1994
- Physical structure and genetic expression of the sulfonamide-resistance plasmid pLS80 and its derivatives in Streptococcus pneumoniae and Bacillus subtilisMolecular Genetics and Genomics, 1984