Evaluation of four commercial enzyme immunoassays for detection of immunoglobulin M antibodies to human parvovirus B19

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Abstract
Four commercial enzyme immunoassays (ElAs) for the detection of parvovirus B19-specific immunoglobulin M (IgM) antibodies [Biotrin Parvovirus B19 IgM (Biotrin International, Ireland); Parvoscan B19 IgM (Euro-Diagnostica, Sweden); Parvovirus IgM (Immunobiological Laboratories [IBL], Germany); and human parvovirus B19 IgM (Hillcrest Biologicals, USA)] were compared to indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR). Using IFA as the reference test, high sensitivities (≥97%) were observed with all four ElAs, though the specificities of the Biotrin and IBL ElAs (99% and 96% respectively) were significantly higher than those of the Hillcrest and Euro-Diagnostica ElAs (81% and 79% respectively).