Cyclic AMP increases incorporation of exogenous fatty acids into triacylglycerols in hamster fibroblasts
- 1 August 1986
- Vol. 21 (8) , 525-528
- https://doi.org/10.1007/bf02535641
Abstract
Incorporation of various exogenous saturated or unsaturated [14C]labeled fatty acids (palmitic, stearic, oleic, linoleic and arachidonic) into triacylglycerols by hamster fibroblasts was markedly enhanced (two‐to fourfold) in the presence of theophylline or dibutyryl cyclic adenosinemonophosphate (dbcAMP). This effect was observed for short‐term (1–6 hr) as well as long‐term (15–24 hr) preincubation with dbcAMP. In the presence of sodium fluoride, a phosphoprotein phosphatase inhibitor, measurement of diacylglycerol acyltransferase (DGAT) activity in cells pretreated with dbcAMP pointed out a marked increase (3X) in specific activity. The results suggest that DGAT activity in fibroblasts could be activated by a cAMP‐dependent phosphorylation process.This publication has 13 references indexed in Scilit:
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