Distribution of Ribosomal Deoxyribonucleic Acid in Subcellular Fractions of Higher Plants
- 1 March 1974
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 53 (3) , 366-369
- https://doi.org/10.1104/pp.53.3.366
Abstract
Methods are described by which ribosomal DNA can be enriched in subcellular fractions of carrot and soybean. With both carrot and cucumber it was possible to obtain a distinct satellite DNA which contained the rDNA. Hybridization values greater than 0.49% were necessary before a satellite component was observed. Saturation hybridization values for soybean, carrot, and cucumber DNA were 0.2, 0.49, and 1.14%, respectively. These values were increased 1.6- and 2-fold in soybean and carrot, respectively, but enrichment was not obtained for cucumber.Keywords
This publication has 9 references indexed in Scilit:
- DNA Complementary to Ribosomal RNA: Relation between Genomic Proportion and PloidyScience, 1973
- Ribosomal RNA Genes and Plant DevelopmentNature, 1972
- Denaturation map of the ribosomal DNA of Xenopus laevisJournal of Molecular Biology, 1971
- The Ribosomal RNA CistronsProgress in Nucleic Acid Research and Molecular Biology, 1971
- Nucleic acid reassociation in formamideBiochemistry, 1969
- Properties and Composition of the Isolated Ribosomal DNA Satellite of Xenopus laevisNature, 1968
- Hybridization of plant ribosomal RNA to DNA: the isolation of a DNA component rich in ribosomal RNA cistrons.Proceedings of the National Academy of Sciences, 1967
- Polyribosome formation and RNA synthesis during aging of carrot-root tissue.Proceedings of the National Academy of Sciences, 1967
- A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membraneJournal of Molecular Biology, 1965