The Failure of Granulocytes to Produce Transcobalamin I (TC I)

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Abstract
The hypothesis that transcobalamin I (TC I) originates from granulocytes (human) was tested by comparing the isoelectric focusing (IEF) patterns of the R-type binder (cobalophilin) of vitamin B12 of: lysed granulocytes, 24 h granulocyte output and plasma. The preparations came from the blood of 5 normal subjects and 4 with myeloproliferative states. The cobalophilin released into a culture of granulocytes resembled the binder in the granulocytes initially. TC I, defined as the .alpha.1 cobalophilin with components isoelectric between pH 2.9-3.35 and as a carrier of native plasma B12, was not released by granulocytes. The granulocyte binder of leukemic granulocytes did not differ from the normal in content per cell or amount released.

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