Bioproduction and purification of rubratoxin B.
- 1 August 1968
- journal article
- Vol. 16 (8) , 1163-7
Abstract
Methods were developed for bioproduction and extraction of rubratoxin B from liquid cultures of Penicillium rubrum P-13 (NRRL A-11785). A maximum of 874.7 mg of toxin per liter of medium was attained in 21 days using stationary cultures of Mosseray's simplified Raulin solution enriched with 2.5% malt extract. Malt extract was required for rubratoxin production. Rubratoxin was not produced in either shake flasks or in fermentors with restricted aeration. Crystalline toxin was obtained by liquid-liquid extraction of concentrated culture medium with ethyl ether. Adhering colored impurities were removed by column chromatography and by recrystallization from acetone.This publication has 6 references indexed in Scilit:
- Rubratoxin B, a toxic metabolite of Penicillium rubrum.1968
- Observations on the structure of the toxins from Penicillium rubrum.1967
- Isolation and characterisation of hepatotoxins from Penicillium rubrumJournal of Pharmacy and Pharmacology, 1966
- The Structures of Mitorubrin and MitorubrinolJournal of the American Chemical Society, 1965
- A DISEASE OF SWINE AND CATTLE CAUSED BY EATING MOLDY CORN .2. EXPERIMENTAL PRODUCTION WITH PURE CULTURES OF MOLDS1957