Transport of B-Vitamins in Microorganisms. IV. The Specificity of the High Accumulation of Labelled Thiamine in Non-proliferating Thiamine Deficient Cells of L. fermenti.

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Abstract
Cells of Lactobacillus fermenti harvested from media with normal and growth limiting levels of thiamln, respectively, were exposed to certain labelled nutrients and the radioactivity retained by the carefully washed cells was measured. Only the permeability to thiamine was increased by the thiamine deficiency, whereas the permeability to adenine, aspartic acid, trypto-phan and niacinamide was virtually not affected and the permeability to glutamic acid and biotin was considerably decreased. Comparative studies were carried out on the uptake of labelled thiamine in non-proliferating cells of L. fermenti and of certain organisms normally not requiring exogenebusly supplied thiamine. The uptake of labelled thiamin in cells of such organisms was much smaller than in L. fermenti and, contrarily to the uptake in L. fermenti. it was practically not affected by exogeneously supplied glucose. Contrarily to what was observed in the case of L. fermenti, the permeability to thiamin of the cells of the other organisms was very little affected by thiamin deficiency in the growth medium.

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