NAD( P)+-dependent hydrogenase activity in extracts from the cyanobacterium Anacystis nidulans

1 January 1996

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 135 (1) , 97-101
https://doi.org/10.1016/0378-1097(95)00436-x

Abstract

Sequence data had indicated that cyanobacteria might possess a bidirectional hydrogenase with properties similar to the soluble enzymes from Alcaligenes eutrophus, Nocardia opaca and Desulfovibrio fructosovorans . The present study shows that extracts from the cyanobacterium Anacystis nidulans catalyse NAD(P)H-dependent H 2 evolution with low but significant activity and uptake of the gas with NAD(P) + as the electron acceptor. NAD + is the preferred electron acceptor and NADH the preferred donor compared to NADP + and NADPH, respectively. Activity levels of this NAD(P) + dependent, bidirectional hydrogenase are too low to support chemoautotrophic growth in A. nidulans .

Keywords

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