SITES OF FORMATION OF IMMUNE GLOBULINS AND OF A COMPONENT OF C'3

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Abstract
The development of a new method for the determination of the sites of serum protein formation was described. The method involved the incorporation of C14-labeled amino acids by tissues cultured in vitro, and subsequent autoradiography of immunoelectrophoretic patterns prepared from a mixture of culture fluids and carrier serum with an antiserum against the carrier serum. This technique was used to demonstrate formation of gamma-globulin, of [beta]2-macroglobulin, and of a component of C3 by mouse spleen tissue, and of various other serum proteins by liver tissue. The specificity and sensitivity of this method was discussed, and some of its advantages and pitfalls mentioned. In addition, a rabbit antimouse serum was prepared, and the immunoelectrophoretic patterns obtained with mouse serum were compared with those described in the literature.