Genetic analysis of protein variations in Mus musculus using two-dimensional electrophoresis
- 1 February 1980
- journal article
- research article
- Published by Springer Nature in Biochemical Genetics
- Vol. 18 (1-2) , 185-197
- https://doi.org/10.1007/bf00504368
Abstract
We have investigated the variation of proteins from crude homogenates of mouse kidneys in several strains of Mus musculus by means of two-dimensional electrophoresis. In this study, we have used the strains C57BL/6J, DBA/2J, CD-1, M. m. castaneus, and M. m. molossinus, as well as offspring from crosses among these strains. Out of the 100 loci screened, we have found nine loci showing interstrain differences. We have been able to identify three proteins as Id-1, Car-2, and Sep-1. The remaining variants are probably new loci in the mouse. Most of the variants (seven) can be mapped to a chromosome. We have found also that differences in the protein pattern as seen on two-dimensional gels are small among subspecies of Mus musculus.Keywords
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