Antibody formation in mouse bone marrow. III. Effects of route of priming and antigen dose.

  • 1 November 1974
    • journal article
    • Vol. 27  (5) , 747-60
Abstract
The influence of the route of priming and the dose of sheep red blood cells (SRBC) on the IgM-, IgG- and IgA-plaque-forming cell (PFC) activity in mouse bone marrow during the secondary response to SRBC was studied. After intraperitoneal and subcutaneous priming the number of IgM-, IgG- and IgA-PFC during the secondary response increased with the number of SRBC used for priming. After intravenous (i.v.) priming the priming dose—secondary response relationship was found to be an optimum curve for IgM-PFC as well as for IgG- and IgA-PFC. A peak secondary response in the bone marrow was found after priming with 107 and with 108 SRBC i.v.