Electron Capture Gas Chromatography of Nitrazepam in Human Plasma as Methyl Derivative
- 1 March 1973
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 6 (3) , 197-210
- https://doi.org/10.1080/00032717308062198
Abstract
A method for quantitative determination of nitrazepam in human plasma in the range 5 - 100 ng/ml is presented. Nitrazepam is extracted with benzene from plasma samples of 0.5 ml, methylated with methyl iodide and determined gas chromatographically with an electron capture detector of 63Ni-type. Acid dissociation constants of nitrazepam are determined and the partition properties studied with benzene, methylene chloride and diethyl ether as organic phases. The selectivity of the method with respect to the metabolites has been thoroughly studied.Keywords
This publication has 4 references indexed in Scilit:
- Determination of nitrazepam (Mogadon) in plasma by electron capture gas-liquid chromatographyJournal of Chromatography A, 1972
- Trimethylsilylation of amino acids derivatization and chromatographyJournal of Chromatography A, 1971
- Ion Pair Extraction in Preparative Organic Chemistry. IV. Alkylation of Methyl Acetoacetate.Acta Chemica Scandinavica, 1969
- High-Sensitivity Detection of Amino-Acids by Gas Chromatography and Electron Affinity SpectrometryNature, 1963