Biosynthesis of porphyrins and corrins. 1. Proton and carbon-13 NMR spectra of (hydroxymethyl)bilane and uroporphyrinogens I and III
- 1 February 1986
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 25 (4) , 896-904
- https://doi.org/10.1021/bi00352a024
Abstract
High-field NMR spectroscopic methods have been applied to study the reactions catalyzed by porphobilinogen (PBG) deaminase and uroporphyrinogen III (uro''gen III) cosynthase, which are the enzymes responsible for the formation of the porphyrin macrocycle. The action of these enzymes in the conversion of PBG, [2,11-13C]PBG, and [3,5-13C]PBG to uro''gens I and III has been followed by 1H and 13C NMR, and assignments are presented. The principal intermediate that accumulated was the correspondingly labeled (hydroxymethyl)bilane (HMB), the assignments for which are also presented.This publication has 1 reference indexed in Scilit:
- Biosynthesis of porphyrins and related macrocycles. Part VI. Nature of the rearrangement process leading to the natural type III porphyrinsJournal of the Chemical Society, Perkin Transactions 1, 1976