Dna Content of Spermatids and Spermatozoa of Haploid and Diploid Drone Honeybees

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Abstract
Testis smears from haploid and diploid pupae were stained with gallocyanin or subjected to the Feulgen reaction. The DNA content of 720 nuclei of spermatids or heads of spermatozoa was measured with an integrating microdensitometer. Twice as much DNA was found in the spermatid nuclei and in the heads of the spermatozoa of diploid drones as in those of the haploids, and it is concluded that the diploid drones produced diploid spermatozoa. No clear evidence was found that diploid drones could produce haploid or tetraploid spermatozoa, or that haploid drones produced diploid spermatozoa.