The Myelin‐Deficient Rat Has a Single Base Substitution in the Third Exon of the Myelin Proteolipid Protein Gene

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Abstract
Several genetic disorders that occur in animals and in humans result in an inability to synthesize normal myelin. Some of these disorders are inherited in an X‐linked manner. The localization of the myelin proteolipid protein (PLP) gene to the X chromosome has directed the study of X‐linked myelination disorders toward PLP. The myelin‐deficient rat is one such X‐linked dysmyelinating mutant. From a cDNA library constructed from myelin‐deficient rat brain mRNA, we have isolated and sequenced cDNAs corresponding to PLP and its alternatively spliced isoform, DM‐20. An A to C transition was detected in these cDNAs, which results in a threonine to proline change at amino acid 74 in both PLP and DM‐20. No other substitutions were seen in the cDNA sequences. Polymerase chain reaction amplification and sequencing of the corresponding genomic regions were used to confirm the single base change. This substitution occurs in a highly hydrophobic portion of the protein that is thought to be an α‐helical transmembrane segment. The presence of a helix‐breaking amino acid such as proline in this segment is likely to influence the ability of the protein to interact with the membrane.