Enzymatic Determination of Dimethylformamide in Waste Water Using Flow Injection Analysis

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Abstract
The novel enzyme N,N-dimethylformamidase has been immobilized on controlled pore glass beads and introduced into a Flow Injection Analysis manifold. Using this system it was possible to determine the dimethylformamide content of industrial waste water and process effluents in 8 of 10 examined samples. Concentrations up to 15mM could be determined with a detection limit of 0.05mM. The immobilized enzyme is stable for months and has an operational lifetime of several days. The analytical system can be used for a permanent control of the dimethylformamide concentration in industrial waste water and process effluents.