Phosphorylation of nitrogen regulator I (NRI) of Escherichia coli.
- 1 December 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (23) , 8919-8923
- https://doi.org/10.1073/pnas.85.23.8919
Abstract
It has previously been shown that phosphorylated nitrogen regulator I (NRi-phosphate) is the activator responsible for increasing the transcription of glnA, the structural gene for glutamine synthetase, and that NRII catalyzes the transfer of the .gamma.-phosphate of ATP to NRI. We have now shown that the reaction of ATP with NRII results in the reversible transfer of the .gamma.-phosphate of ATP to a histidine residue of NRII. In turn, NRII-phosphate transfer its phosphate reversibly to an aspartic residue of NRI-phosphate is hydrolyzed to NRI and inorganic phosphate in a divalent cation-requiring autocatalytic reaction.This publication has 24 references indexed in Scilit:
- Crosstalk between bacterial chemotaxis signal transduction proteins and regulators of transcription of the Ntr regulon: evidence that nitrogen assimilation and chemotaxis are controlled by a common phosphotransfer mechanism.Proceedings of the National Academy of Sciences, 1988
- Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxisCell, 1988
- Sensory transduction in bacterial chemotaxis involves phosphotransfer between CHE proteinsBiochemical and Biophysical Research Communications, 1988
- Protein phosphorylation is involved in bacterial chemotaxis.Proceedings of the National Academy of Sciences, 1987
- Techniques in the detection and characterization of phosphoramidate-containing proteinsPublished by Elsevier ,1984
- Chemical properties, isolation, and analysis of O-phosphates in proteinsPublished by Elsevier ,1984
- Chapter 15. Chromosomal Protein Phosphorylation on Basic Amino AcidsPublished by Elsevier ,1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The preparation and characterization of phosphorylated derivatives of histidineBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1968
- The Preparation and Characterization of 1-Phosphohistidine and 3-Phosphohistidine*Biochemistry, 1966