Stimulated DNA Synthesis in Frog Nuclei by Cytoplasmic Extracts of Temperature-Sensitive Mammalian Cells
- 18 August 1978
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 201 (4356) , 651-652
- https://doi.org/10.1126/science.675253
Abstract
Cytoplasmic extracts of proliferating cells stimulate DNA synthesis in isolated nuclei of Xenopus laevis liver. When tested by the same assay, cytoplasmic extracts of resting cells are completely inactive. When cytoplasmic extracts are prepared from cell cycle-specific temperature-sensitive mutants arrestd in the G1 phase of the cell cycle by the nonpermissive temperature, they also fail to stimulate DNA synthesis in frog nuclei. The results indicate that, to stimulate DNA synthesis in isolated frog nuclei, essentially all information of G1 cells must be present.This publication has 13 references indexed in Scilit:
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