Detection of Antilymphocyte Antibody with Two-Color Method in Systemic Lupus Erythematosus and Its Heterogeneous Specificities against Human T-Cell Subsets
Open Access
- 1 November 1979
- journal article
- research article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 64 (5) , 1213-1220
- https://doi.org/10.1172/jci109575
Abstract
The two-color method originally described by Van Rood et al. (Van Rood, J. J., A. Van Leeuwen, and J. S. Ploen. 1976. Simultaneous detection of two cell populations by two-color fluorescence and application to the recognition of B-cell determinants. Nature (Lond.). 262: 795-797) for the typing of homologous leukocytic antibodies, D-region was used for the detection of antilymphocyte antibody (ALA) in systemic lupus erythematosus. In this method, surface immunoglobulin-bearing cells were identified with fluorescein isothiocyanate-labeled anti-immunoglobulin and nuclei of killed cells were stained with ethidium bromide. Therefore, cell type (T or B) of the target cells can be identified without fractionating them. ALA was detected in 87% of lupus sera and had a preferential reactivity with T cells. Its major immunoglobulin class was shown to be immunoglobulin (Ig)M. The subspecificity of ALA was further analyzed using fractionated T-cell subsets as target cells. When T lymphocytes were separated into Fc receptor-bearing (Tγ) and lacking (Tγ[-]) cells, 64% of ALA showed preferential reactivity with Tγ cells and 14% with Tγ(-) cells. The remainder had no selective reactivity against Tγ or Tγ(-) cells. Tγ cells were shown to have suppressor activity, whereas Tγ(-) cells were indicated to contain helper cells. The above finding was in agreement with the observation that treatment of T cells with ALA that preferentially react with Tγ cells considerably enhanced immunoglobulin synthesis in vitro, whereas treatment of T cells with ALA reactive with Tγ(-) cells clearly suppressed the formation of immunoglobulins. Treatment of ALA with no selective reactivity showed variable effects on in vitro immunoglobulin synthesis. These results indicate that ALA in lupus have heterogeneous specificities against human T-cell subsets.This publication has 33 references indexed in Scilit:
- Differential sensitivity of functional subsets of t cells to the cytotoxicity of natural t‐lymphocytotoxic autoantibody of systemic lupus erythematosusArthritis & Rheumatism, 1979
- Suppressor-Cell Dysfunction in Systemic Lupus ErythematosusJournal of Clinical Investigation, 1978
- A serum inhibitor of immune regulation in patients with systemic lupus erythematosus.Journal of Clinical Investigation, 1978
- Fc-Receptor Heterogeneity of Human Suppressor T CellsThe Journal of Immunology, 1978
- Studies of immune functions of patients with systemic lupus erythematosus. i. dysfunction of suppressor t‐cell activity related to impaired generation of, rather than response to, suppressor cellsArthritis & Rheumatism, 1978
- Isolation and Characterization of Naturally Occurring Subclasses of Human Peripheral Blood T Cells with Regulatory FunctionsThe Journal of Immunology, 1978
- Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG.The Journal of Experimental Medicine, 1977
- Suppressor T-cell abnormality in idiopathic systemic lupus erythematosusClinical Immunology and Immunopathology, 1976
- Antibodies for T lymphocytes in systemic lupus erythematosus.1976
- Microdroplet Assay of Human Serum CytotoxinsNature, 1964