Accurate transcription of homologous 5S rRNA and tRNA genes and spllcing of tRNAin vitroby soluble extracts ofNeurospora
Open Access
- 25 July 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (14) , 5737-5755
- https://doi.org/10.1093/nar/12.14.5737
Abstract
We have developed soluble extracts from Neurospora crassa capable of accurately and efficiently transcribing homologous 5S rRNA and tRNA genes. The extracts also appear to quantitatively end-process and splice the primary tRNA transcripts. Although the extracts could not transcribe a heterologous (yeast) 5S rRNA gene, they did transcribe a yeast tRNALeu gene and slowly process the transcripts. In addition, we have developed a novel strategy for rapidly sequencing uniformly labelled RNAs using base-specific ribonucleases. We have used this procedure to verify the identity of the in vitro tran scripts and processing products.Keywords
This publication has 39 references indexed in Scilit:
- Cis-acting and trans-acting regulatory mutations define two types of promoters controlled by the qa-1F gene of NeurosporaCell, 1984
- Nucleotide sequence of yeast LEU2 shows 5′-noncoding region has sequences cognate to leucineCell, 1982
- Dispersed 5S RNA genes in N. crassa: Structure, expression and evolutionCell, 1981
- Promoter Sequences of Eukaryotic Protein-Coding GenesScience, 1980
- Order and intracellular location of the events involved in the maturation of a spliced tRNANature, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the regionCell, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the regionCell, 1980
- Selective and accurate initiation of transcription at the ad2 major late promotor in a soluble system dependent on purified rna polymerase ii and dnaCell, 1979
- A nuclear extract of xenopus laevis oocytes that accurately transcribes 5S RNA genesCell, 1978
- New rapid gel sequencing method for RNANature, 1977