Identification and characterization of a new transcriptional termination factor from Escherichia coli.

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Abstract
An activity from Escherichia coli that enhances transcription termination at the bacteriophage T7 early terminator when cloned on the plasmid pAR1707 was identified and partially purified. The factor also causes the transcript to be terminated at a site several nucleotides earlier than in its absence. The resulting 3'' OH ends of the transcripts are identical to those found in vivo by S1 nuclease mapping. This factor is probably responsible for determination of the 3'' OH ends of 17 RNA transcripts in vivo. It does no act by processing a performed RNA transcript, nor is it replaced by rho protein or nusA or nusB proteins. Therefore, it appears to be a new transcription termination factor, which is designated "tau factor". Elucidation of its role in transcription in E. coli will depend on its purification to homogeneity and further studies of its properties.