Phosphate uptake in chemostat cultures of Escherichia coli K-12 subjected to periodic β-glycerophosphate pulsing: a system for assaying alkaline phosphatase
- 1 May 1979
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 25 (5) , 560-564
- https://doi.org/10.1139/m79-081
Abstract
Limiting concentrations of β-glycerophosphate were pulsed into chemostat cultures of Escherichia coli K-12 at intervals equal to the population doubling time. The resultant culture density fluctuations are interpreted in terms of inorganic phosphate uptake which, in this system, is a function of alkaline phosphatase activity. Information concerning in vivo alkaline phosphatase activity at suboptimal (acidic) pH with very low concentrations of substrate (β-glycerophosphate) is obtained from kinetic analysis of uptake data.Keywords
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